Journal:

Article Title: Fibrosis in left atrial tissue of patients with atrial fibrillation with and without underlying mitral valve disease

doi: 10.1136/hrt.2003.015347

Figure Lengend Snippet: Sirius red staining of left atrial tissue for connective tissue (red staining) from patients (A) in sinus rhythm (SR) and (B) with chronic atrial fibrillation (lone CAF). Immunohistological results in left atrial tissue from (left) patients in SR and (right) patients with lone CAF for (C, D) collagen type I, (E, F) collagen type III, and (G, H) fibronectin. Original magnification ×400.

Article Snippet: Goat anti-human collagen I, goat anti-human collagen III (Santa Cruz Biotechnology) and rabbit anti-human fibronectin (DPC Biermann, Bad Nauheim, Germany) were used as primary antibodies.

Techniques: Staining

Journal:

Article Title: Fibrosis in left atrial tissue of patients with atrial fibrillation with and without underlying mitral valve disease

doi: 10.1136/hrt.2003.015347

Figure Lengend Snippet: (A) Collagen I, collagen III, and fibronectin expression in atrial tissue of (left columns) patients in SR (left columns) and (right columns) patients with atrial fibrillation. (B) Comparison of all patients in the study either (left columns) with underlying mitral valve disease (MVD) or (right columns) without MVD.

Article Snippet: Goat anti-human collagen I, goat anti-human collagen III (Santa Cruz Biotechnology) and rabbit anti-human fibronectin (DPC Biermann, Bad Nauheim, Germany) were used as primary antibodies.

Techniques: Expressing, Comparison

Journal:

Article Title: Fibrosis in left atrial tissue of patients with atrial fibrillation with and without underlying mitral valve disease

doi: 10.1136/hrt.2003.015347

Figure Lengend Snippet: (A) Collagen I, (B) collagen III, and (C) fibronectin expression in left atrial tissue of patients in SR, with lone AF, and with AF with underlying MVD (MVD+AF).

Article Snippet: Goat anti-human collagen I, goat anti-human collagen III (Santa Cruz Biotechnology) and rabbit anti-human fibronectin (DPC Biermann, Bad Nauheim, Germany) were used as primary antibodies.

Techniques: Expressing

Journal:

Article Title: Fibrosis in left atrial tissue of patients with atrial fibrillation with and without underlying mitral valve disease

doi: 10.1136/hrt.2003.015347

Figure Lengend Snippet: Quantitative analysis of (A) collagen I, (B) collagen III, and (C) fibronectin expression in human atrial tissue of the SR, paroxysmal atrial fibrillation (PAF), and CAF subgroups of lone AF and MVD+AF.

Article Snippet: Goat anti-human collagen I, goat anti-human collagen III (Santa Cruz Biotechnology) and rabbit anti-human fibronectin (DPC Biermann, Bad Nauheim, Germany) were used as primary antibodies.

Techniques: Expressing

Journal: Molecular Vision

Article Title: Downregulation of LIM kinase 1 suppresses ocular inflammation and fibrosis

doi:

Figure Lengend Snippet: LIMK1 siRNA downregulates LIMK1 protein and suppresses fibronectin deposition in human corneal fibroblasts. A : western blot analysis. Corneal fibroblasts were transfected as indicated for 72 h. Cells were lysed and protein levels were analyzed using LIMK1 and GAPGH antibodies. B-E : Immunofluorescence microscopy using fibronectin antibody and DAPI staining in fibroblast cultures ( B ) untreated; ( C ) treated with 100 nM scrambled control siRNA; ( D ) treated with 50 nM of LIMK1 -targeted siRNA; or ( E ) treated with 100 nM of LIMK1 -targeted siRNA. F : Quantitative analysis of fibronectin secreted to the culture media by western blotting. Samples were untreated or treated with control or LIMK1 -targeted siRNA as indicated. Experiments were performed 3 times yielding similar results. Error bars represent standard deviation. The asterisk indicates a p<0.025 (n=3) compared to scrambled siRNA.

Article Snippet: To carry out immunostaining, corneal fibroblasts were incubated with polyclonal rabbit anti-human fibronectin (1:100; BD Biosciences, Lexington, KY) or monoclonal anti-paxillin (1:100; Millipore, Billercia, MA).

Techniques: Western Blot, Transfection, Immunofluorescence, Microscopy, Staining, Standard Deviation

Journal: EMBO Molecular Medicine

Article Title: ENOblock synergizes with colistin to treat Acinetobacter baumannii infections

doi: 10.1038/s44321-025-00331-2

Figure Lengend Snippet: ( A ) Schematic of the bacterial adhesion/invasion assay. ( B ) Analysis of Ab ATCC 17978 and Ab CR17 strains adhesion into HeLa and macrophage cells with (1xMIC) and without ENOblock treatment. The data are presented as means of three biological replicates ± SEM, For HeLa cells, * P = 0.023: Ab ATCC 17978 vs Ab ATCC 17978 + ENOblock and * P = 0.048: Ab CR17 vs Ab CR17 + ENOblock (two-tailed Student’s t test). For macrophage cells, * P = 0.018: Ab ATCC 17978 vs Ab ATCC 17978 + ENOblock and * P = 0.035: Ab CR17 vs Ab CR17 + ENOblock (two-tailed Student’s t test). ( C ) Analysis of Ab ATCC 17978 and Ab CR17 strains invasion into HeLa and macrophage cells with (1×MIC) and without ENOblock treatment. The data are presented as means of three biological replicates ± SEM, For HeLa cells, * P = 0.001: Ab ATCC 17978 vs Ab ATCC 17978 + ENOblock and * P = 0.011: Ab CR17 vs Ab CR17 + ENOblock (two-tailed Student’s t test). For macrophage cells, * P = 0.001: Ab ATCC 17978 vs Ab ATCC 17978 + ENOblock and * P = 0.002: Ab CR17 vs Ab CR17 + ENOblock (two-tailed Student’s t test). ( D ) Immunostaining of fibronectin of HeLa cells (magenta) and Ab ATCC 17978 and Ab CR17 strains (green) pretreated with ENOblock (0× and 1×MIC), after bacterial adherence for 2 h, was performed by specific primary antibodies against both strains and their respective secondary antibodies. Blue staining shows the location of HeLa cell nuclei. A representative image out of three biological replicates is shown. .

Article Snippet: Rabbit anti-human fibronectin , Merck , Cat# F3648.

Techniques: Invasion Assay, Two Tailed Test, Immunostaining, Staining

Journal: EMBO Molecular Medicine

Article Title: ENOblock synergizes with colistin to treat Acinetobacter baumannii infections

doi: 10.1038/s44321-025-00331-2

Figure Lengend Snippet: ( A – C ) Consensus-spectrum (CIS) of enolase, plasminogen and ENOblock, enolase, fibronectin and ENOblock, and enolase, fibrinogen and ENOblock. ( D – F ) Structural models of protein-protein complex generated by Alphafold3 analysis by docking of ENOblock (ball and stick), enolase (pelorous ribbons) and plasminogen, fibronectin or fibrinogen (tangerine ribbons). The intermolecular interactions from the obtained complex were according to ISM method–detected interaction domains (yellow ribbons). ( G – I ) Inhibition of Ab adherence to immobilized plasminogen, fibronectin and fibrinogen by free enolase. Ab ATCC 17978 strain was incubated in plasminogen, fibronectin and fibrinogen-coated wells for 3 h at room temperature, containing increasing concentrations of free enolase (0, 10, 50 and 100 mg/L). The data are presented as means of three biological replicates ± SEM. For plasminogen, * P = 0.023: Ab ATCC 17978 vs Ab ATCC 17978 + enolase (10 mg/L), * P = 0.002: Ab ATCC 17978 vs Ab ATCC 17978 + enolase (50 mg/L) and * P = 0.001: Ab ATCC 17978 vs Ab ATCC 17978 + enolase (100 mg/L) (two-tailed Student’s t test). For fibronectin, * P = 0.006: Ab ATCC 17978 vs Ab ATCC 17978 + enolase (10 mg/L), * P = 0.025: Ab ATCC 17978 vs Ab ATCC 17978 + enolase (50 mg/L) and * P = 0.005: Ab ATCC 17978 vs Ab ATCC 17978 + enolase (100 mg/L) (two-tailed Student’s t test). For fibrinogen, * P = 0.039: Ab ATCC 17978 vs Ab ATCC 17978 + enolase (10 mg/L), * P = 0.017: Ab ATCC 17978 vs Ab ATCC 17978 + enolase (50 mg/L) and * P = 0.002: Ab ATCC 17978 vs Ab ATCC 17978 + enolase (100 mg/L) (two-tailed Student’s t test). ( J – L ) Inhibition of Ab adherence to immobilized plasminogen, fibronectin and fibrinogen by ENOblock. Ab ATCC 17978 strain was incubated in plasminogen, fibronectin and fibrinogen-coated wells for 2 h at room temperature, containing increasing concentrations of ENOblock (0.5× and 1×MIC). The data are presented as means of three biological replicates ± SEM, * P < 0.05: treatment vs no treatment; two-tailed Student’s t test. For plasminogen, * P = 0.039: Ab ATCC 17978 vs Ab ATCC 17978 + ENOblock (0.5×MIC) and * P = 0.008: Ab ATCC 17978 vs Ab ATCC 17978 + ENOblock (1xMIC) (two-tailed Student’s t test). For fibronectin, * P = 0.026: Ab ATCC 17978 vs Ab ATCC 17978 + ENOblock (0.5×MIC) and * P = 0.042: Ab ATCC 17978 vs Ab ATCC 17978 + ENOblock (1×MIC) (two-tailed Student’s t test). For fibrinogen, * P 0.022 = Ab ATCC 17978 vs Ab ATCC 17978 + ENOblock (0.5×MIC) and * P = 0.045: Ab ATCC 17978 vs Ab ATCC 17978 + ENOblock (1xMIC) (two-tailed Student’s t test). Adherent bacteria to plasminogen, fibronectin and fibrinogen-coated wells were quantified by serial dilutions as described in materials and methods. Results were expressed as the percentage of total untreated Ab adhered to immobilized plasminogen, fibronectin and fibrinogen. .

Article Snippet: Rabbit anti-human fibronectin , Merck , Cat# F3648.

Techniques: Generated, Inhibition, Incubation, Two Tailed Test, Bacteria

Journal: Hepatology (Baltimore, Md.)

Article Title: Human skin fibroblasts: From mesodermal to hepatocyte-like differentiation.

doi: 10.1002/hep.21839

Figure Lengend Snippet: Fig. 1. Comparative fibroblasts and MSCs characterization. Morphological aspect of fibroblasts (A, day 13) and MSCs (B, day 15) in first passage growth culture. Immunofluorescence assay showing stainings of fibroblasts (C-F) and MSCs (G-J) for vimentin (C,G), fibronectin (D, H), ASMA (E,I) and laminin (F,J) (n 3 each). Absence of CD200 expression in fibroblasts (K) as shown by immunocytofluorescence assay whereas this marker was positively detected in human neuroblastoma SH-SY5Y cells (n 3). Nuclei were revealed by DAPI staining. Pictures magnifications are (A-J) 400 and (K,L) 200. (M) Example of representative flow cytometric profile of fibroblasts at first passage. Analyzed epitopes are indicated in the histograms. Bars indicate the fluorescence level of corresponding isotype. (N) Comparative cytometric phenotype of MSCs and human skin fibroblasts (both cells from passages 2 to 4). Fibroblasts are indicated by (-f) suffixes.

Article Snippet: Polyclonal anti-human antibodies were incubated overnight at RT at the following concentrations: 1/3000 rabbit anti-human fibronectin, 1/1000 rabbit anti-human FP and 1/200 guinea pig anti-human CK18 (Progen).

Techniques: Expressing, Marker, Staining

Journal: Hepatology (Baltimore, Md.)

Article Title: Human skin fibroblasts: From mesodermal to hepatocyte-like differentiation.

doi: 10.1002/hep.21839

Figure Lengend Snippet: Fig. 8. In vivo hepatocyte differentiation of human skin fibroblasts. Pictures show immunohistochemical stains for human markers fibronectin, FP, albumin, and CK18. Analysis of livers of transplanted SCID-mice revealed that fibroblasts engrafted as small clusters of cells presenting combined expression of mesenchymal marker fibronectin and liver-specific markers FP, albumin, and CK18. Positive and negative control stains are presented, respectively, on human and mouse livers. Abbreviations: FP, alpha-1-fetoprotein. Pictures were taken at 400 magnification.

Article Snippet: Polyclonal anti-human antibodies were incubated overnight at RT at the following concentrations: 1/3000 rabbit anti-human fibronectin, 1/1000 rabbit anti-human FP and 1/200 guinea pig anti-human CK18 (Progen).

Techniques: In Vivo, Immunohistochemical staining, Expressing, Marker, Negative Control